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insta-blot-breast-tissue-oncopair  (Novus Biologicals)


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    Novus Biologicals insta-blot-breast-tissue-oncopair
    Insta Blot Breast Tissue Oncopair, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oncopair+insta/us10739344-486-0-0?v=Novus+Biologicals
    Average 90 stars, based on 1 article reviews
    insta-blot-breast-tissue-oncopair - by Bioz Stars, 2026-07
    90/100 stars

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    Fig. 1 Upregulation of SHC1 p46SHC/p52SHC transcript and SHC proteins in human breast cancers. a Quantitative real-time RT-PCR analysis of SHC1 p52SHC/p46SHC transcript levels on a TissueScan cancer survey panel of the breast, colon, prostate, lung, ovary, thyroid, kidney, and liver non-tumor tissue (red bars) and tumor tissue (black bars). Expression is normalized to the median non-cancerous tissue samples. Expression of p52SHC/p46SHC transcript greater than 1.5 is indicated with a number sign, while expression greater than 2 times is indicated with an asterisk. b Western blot analysis of SHC1 isoform expression of paired ER+/PR+/HER2−tumor (T) and non-tumor (N) samples, 5 μg/lane. Lane numbers are indicated at the bottom. c Quantification of data from b panel by normalization to total protein, where *P = 0.008. d Western blot analysis of SHC1 in triple negative ER −/PR−/HER2−paired samples, 10 μg/lane. Lane numbers are indicated at the bottom. e Quantification of data from d panel by normalization to total protein. f INSTA-Blot Breast Tissue OncoPair membrane blotted with both c-terminal total SHC1 and n-terminal p66SHC-specific antibodies. Membranes contain seven paired tumor (T) or non-tumor (N) samples. Locations of three SHC isoforms are indicated. g Western blot analysis of SHC isoform expression (red bands) and β-actin (green) as a loading control in paired normal (N) and DMBA tumor (T) samples from the same rats (n = 3 rats)

    Journal: Breast cancer research : BCR

    Article Title: The p52 isoform of SHC1 is a key driver of breast cancer initiation.

    doi: 10.1186/s13058-019-1155-7

    Figure Lengend Snippet: Fig. 1 Upregulation of SHC1 p46SHC/p52SHC transcript and SHC proteins in human breast cancers. a Quantitative real-time RT-PCR analysis of SHC1 p52SHC/p46SHC transcript levels on a TissueScan cancer survey panel of the breast, colon, prostate, lung, ovary, thyroid, kidney, and liver non-tumor tissue (red bars) and tumor tissue (black bars). Expression is normalized to the median non-cancerous tissue samples. Expression of p52SHC/p46SHC transcript greater than 1.5 is indicated with a number sign, while expression greater than 2 times is indicated with an asterisk. b Western blot analysis of SHC1 isoform expression of paired ER+/PR+/HER2−tumor (T) and non-tumor (N) samples, 5 μg/lane. Lane numbers are indicated at the bottom. c Quantification of data from b panel by normalization to total protein, where *P = 0.008. d Western blot analysis of SHC1 in triple negative ER −/PR−/HER2−paired samples, 10 μg/lane. Lane numbers are indicated at the bottom. e Quantification of data from d panel by normalization to total protein. f INSTA-Blot Breast Tissue OncoPair membrane blotted with both c-terminal total SHC1 and n-terminal p66SHC-specific antibodies. Membranes contain seven paired tumor (T) or non-tumor (N) samples. Locations of three SHC isoforms are indicated. g Western blot analysis of SHC isoform expression (red bands) and β-actin (green) as a loading control in paired normal (N) and DMBA tumor (T) samples from the same rats (n = 3 rats)

    Article Snippet: Human INSTA-BlotTM Breast Tissue OncoPair (Novus Biologicals #NBP2-30128) ready-to-use PVDF membranes were used to assess total SHC proteins expressions in human breast cancer and normal adjacent tissue, 14 μg total protein per sample.

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Membrane, Control

    Figure 1: Prdx Expression in Tumor (T) and Adjacent Normal (N) Tissue. (A) Oncopair INSTAblot IMB-130d, including matched tumor and adjacent non- tumor breast tissue for 7 patient donors. Tumor diagnosis for patients are as follows: P1, mucinous carcinoma; P2, mucinous carcinoma; P3, mucinous carcinoma; P4, metaplastic; P5, intraductal carcinoma; P6, intraductal carcinoma; P7, intraductal carcinoma; (B) Oncopair INSTAblot IMB-130a, including matched tumor and adjacent non -tumor breast tissue for 7 patient donors. All breast tumors were diagnosed as ductal carcinoma. (*note – the patient orientation of this blot is reversed from the published order, based on a difference in loading order for this blot lot).

    Journal: International Journal of Cancer and Clinical Research

    Article Title: Prdx Overexpression in Tumor Tissue of Breast Cancer Patients

    doi: 10.23937/2378-3419/3/2/1053

    Figure Lengend Snippet: Figure 1: Prdx Expression in Tumor (T) and Adjacent Normal (N) Tissue. (A) Oncopair INSTAblot IMB-130d, including matched tumor and adjacent non- tumor breast tissue for 7 patient donors. Tumor diagnosis for patients are as follows: P1, mucinous carcinoma; P2, mucinous carcinoma; P3, mucinous carcinoma; P4, metaplastic; P5, intraductal carcinoma; P6, intraductal carcinoma; P7, intraductal carcinoma; (B) Oncopair INSTAblot IMB-130a, including matched tumor and adjacent non -tumor breast tissue for 7 patient donors. All breast tumors were diagnosed as ductal carcinoma. (*note – the patient orientation of this blot is reversed from the published order, based on a difference in loading order for this blot lot).

    Article Snippet: Two OncoPair INSTA-Blots membranes were purchased from Imgenex/Novus (San Diego, CA).

    Techniques: Expressing, Biomarker Discovery